Histology Guide

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MICROSCOPE SLIDE

SLIDE NAME

MH 016 Simple Epithelia

TISSUE

Trachea (monkey)
Small Intestine (monkey)
Ovary (human)
Kidney (human)

STAIN

Hematoxylin & Eosin

FIXATIVES

Zenker's Formaldehyde
(trachea, small intestine, kidney)
4% Formaldehyde
(ovary)

IMAGE SIZE

147,440 x 32,102 pixels
17.6 GB

FILE SIZE

303 MB

MAGNIFICATION

40x

PIXEL SIZE

0.3171 µm

SOURCE

Medical School
University of Minnesota
Minneapolis, MN

CONTACT

Questions or comments should be sent to
tcbrelje@gmail.com

This web site is owned and operated by:

T. Clark Brelje, Ph.D.

Faculty/Retired
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Version 5.0

Copyright © 2005-2021
T. Clark Brelje and Robert L. Sorenson
All rights reserved.

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MH 016 Simple Epithelia

Pseudostratified Columnar Epithelium

The is a tube that connects the larynx to the lungs allowing the passage of air. It is lined with a

As its name implies, this epithelium appears to be stratified (i.e., has multiple layers of cells) but in fact all of the cells are attached to the basement membrane. Therefore, it is a simple epithelium. The stratified appearance is due to the nuclei of individual cells being present at different levels.

Features of this :

Cilia - extend 5 to 7 µm from the surface of the epithelial cells. The dark line at their base is from their basal bodies.
Goblet Cells - scattered cells that secrete mucus. They are difficult to identify in this specimen, but a covers some regions of the epithelium.
Basement Membrane - the epithelium is separated from the underlying connective tissue by a thick basement membrane. It is seen as an acidophilic band beneath the epithelium.

Since it lines the respiratory tract, a pseudostratified ciliated, columnar epithelium with goblet cells is referred to as the "respiratory epithelium".

The ultrastructure of these epithelial cells is also shown in EM 070 Respiratory Epithelium by transmission electron microscopy.