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CHAPTER 7 - PERIPHERAL BLOOD
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 033hr Blood Smear
TISSUE
Blood
(human)
STAIN
Wright's Stain
(mixture of methylene blue,
azure II, and eosin)
FIXATIVE
Air Dry
Methanol
IMAGE SIZE
15,387 x 14,892 pixels
854 MB
FILE SIZE
50 MB
OBJECTIVE
60x
(oil immersion objective)
PIXEL SIZE
0.0956 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 033hr Blood Smear

Identify which group is red blood cells:

(,,)
(,,)
(,,)
(,,)
(,)

What is the diameter of red blood cells (RBC)? [ + ]

7 to 8 µm

Do RBC have nuclei in humans? [ + ]

No

Why is the cytoplasm of RBC so eosinophilic? [ + ]

High concentration of the protein hemoglobin

Why does the center of RBC appear more lightly stained? [ + ]

RBC are biconcave discs - their thickness at the center is much less than on the edges

How can RBC pass through capillaries as small as 4 µm in diameter? [ + ]

RBC are capable of dynamic shape change that allows them to "fold" and pass through small capillaries

What is the primary function of RBC? [ + ]

Transport of oxygen and carbon dioxide

What is the lifespan of RBC? [ + ]

~120 days - phagocytosed by macrophages

Identify which group is platelets:

(,)
(,,)
(,,)
(,,)
(,,)

What is the approximate diameter of platelets? [ + ]

2 µm

What cells give rise to platelets? [ + ]

Megakaryocytes

What is the primary function of platelets? [ + ]

Formation of blood clots (i.e.,, hemostatic plug)
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