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CHAPTER 7 - PERIPHERAL BLOOD
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 033hr Blood Smear
TISSUE
Blood
(human)
STAIN
Wright's Stain
(mixture of methylene blue,
azure II, and eosin)
FIXATIVE
Air Dry
Methanol
IMAGE SIZE
15,387 x 14,892 pixels
854 MB
FILE SIZE
50 MB
OBJECTIVE
60x
(oil immersion objective)
PIXEL SIZE
0.0956 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 033hr Blood Smear

Identify wich group is neutrophils:

(,,)
(,)
(,,,)
(,,)
(,,)

What is another name for neutrophils? [ + ]

Polymorphonuclear leukocytes (PMN)

What is the size of neutrophils? [ + ]

12 to 15 µm

Describe the nucleus of a neutrophil: [ + ]

Segmented with 2 to 5 lobes containing clumped chromatin

Do neutrophils contain specific granules? [ + ]

Yes, weakly stained neutral to light lilac/peach granules

Do neutrophils contain azurophilic granules [ + ]

Yes, small purple granule that are less numerous than specific granules

What is the primary function of neutrophils [ + ]

  • Leave the blood stream at sites of infection or inflammation
  • Phagocytose and destroy bacteria and other infectious agents

Identify which group is eosinophils:

(,,)
(,,)
(,,,)
(,,)
(,)

What is the size of eosinophils? [ + ]

12 to 18 µm in diameter (slightly larger than neutrophils)

Describe the nucleus of an eosinophil: [ + ]

Typically, bi-lobed

Do eosinophils contain specific granules? [ + ]

Yes, intensely stained large pink/orange eosinophilic granules

Do neutrophils contain azurophilic granules? [ + ]

Yes, typically only a few dark purple granules

What is the function of eosinophils? [ + ]

  • Leave the blood stream at sites of infection or inflammation
  • Role in killing multicellular parasites
  • Moderate inflammatory responses by phagocytosis of immune complexes

Identify which group is basophils:

(,,,)
(,,)
(,,)
(,,)
(,)

What is the size of basophils? [ + ]

10 to 12 µm in diameter (same size as neutrophils)

Describe the nucleus of a basophil: [ + ]

Bi-lobed, S-shaped, or indented

Do basophils contain specific granules? [ + ]

Yes, intensely stain blue/purple granules

Do basophils contain azurophilic granules? [ + ]

Yes, typically only a few (cannot be distinguished from specific granules)

What is the function of basophils? [ + ]

  • Basophils have a role in immediate (bronchial asthma) and delayed (allergic skin reaction) hypersensitivity
  • Basophils have IgE receptors on their surface that bind antigens which triggers the release of potent vasoactive substances
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