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CHAPTER 2 - EPITHELIUM
Histology Guide
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MICROGRAPH

NAME
EM 070 Respiratory Epithelium
TISSUE
Trachea
(human)
IMAGE SIZE
4,629 x 5,225 pixels
92 MB
FILE SIZES
31,014 KB (grayscale)
31,092 KB (color)
MAGNIFICATION
9,400x
PIXEL SIZE
2.360 nm
SOURCE
Dave Muchow
Anna Marie Carpenter
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

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University of Minnesota
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EM 070 Respiratory Epithelium

Pseudostratified Columnar Epithelium

Although a pseudostratified columnar epithelium is a single layer of cells, the nuclei are not aligned in the same plane. This can give the appearance of several layers of cells.

The pseudostratified columnar cells with cilia and goblet cells found in airways is also known as respiratory epithelium.

This thin section is from the trachea but no goblet cells are seen in this micrograph. The base of the ciliated cell is not visible.

  • Nuclei (blue) / Nuclear Envelope (purple)
  • Mitochondria (red)
  • Endoplasmic Reticulum (cyan)
  • Cytoplasm (green)
  • (green) - outer nine doublets and central pair of singlet microtubules are seen in of cilia
  • (purple) - thin, fuzzy line that separates the epithelium from the underlying connective tissue. It contains a network of reticular, collagen IV fibrils.

An immune cell (tan) can be seen at the bottom of the epithelium. (Lymphocytes can cross the basal lamina and move into an epithelium.)

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