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CHAPTER 3 - CONNECTIVE TISSUE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 003 Toluidine Blue
TISSUE
Pancreas (rat)
Lymph Node (rat)
Cerebellum (rat)
Liver (rat)
STAIN
Toluidine Blue
FIXATIVE
Bouin's Solution
IMAGE SIZE
93,145 x 66,275 pixels
23 GB
FILE SIZE
5.39 GB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 003 Toluidine Blue

Examine the staining of the surrounding this lymph node:

What dye(s) were used to stain this tissue section? [ + ]

Toluidine blue

What is staining a different color than the dye known as? [ + ]

Metachromasia

What cells demonstrate this in connective tissue? [ + ]

Mast cells - their secretion granules are stained dark purple and obscure the nuclei

Why does this occur? [ + ]

Intense staining due to the high concentration of negative charges found in heparin contained in these granules
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