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CHAPTER 1 - THE CELL
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 015 Mitosis
TISSUE
Onion Root Tip
STAIN
Iron Hematoxylin
FIXATIVE
Flemming's Solution
(chromic acid, osmic acid,
and acetic acid)
IMAGE SIZE
48,000 x 36,000
5.2 GB
FILE SIZE
1.07 GB
OBJECTIVE
60x
(oil immersion objective)
PIXEL SIZE
0.1500 µm
SOURCE
Medical School
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

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University of Minnesota
Department of Genetics, Cell Biology and Development
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Minneapolis, MN 55455

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MH 015 Mitosis

Mitosis
(Iron Hematoxylin)

An onion root tip is a rapidly growing part of an onion and thus many cells will be in different stages of mitosis. An onion cell possesses 8 chromosomes (whereas human cells possess 46 chromosomes).

Examine the following cells in different stages of mitosis. (Observe the cell at the center of each view.)

  • Interphase (,, and ) - non-dividing cells (majority of the cells)
  • Prophase (, , and ) - condensation of chromosomes but not organized
  • Metaphase (and ) - chromosomes line up in the center of the cell
  • Anaphase (,, and ) - chromosomes are pulled to opposite sides of the cells
  • Telophase (and ) - chromosomes arrive at the cell poles and will form two nuclei

Practice locating other cells in each stage of mitosis.

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