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CHAPTER 15 - LIVER AND GALLBLADDER
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 031 Liver
TISSUE
Liver
Kidney
(mouse)
STAIN
Trypan Blue Uptake
(injected with 0.5% trypan blue
subcutaneously for 5 days)
(eosin counterstain)
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZE
38,800 x 28,769 pixels
4.2 GB
FILE SIZE
118 MB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

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University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 031 Liver

Liver

Kupffer cells are fixed macrophages found in the walls of hepatic sinusoids.

This animal was injected with trypan blue. For most cells, trypan blue is a vital dye. It is excluded from living cells and enters into and stains dead cells. However, trypan blue is also taken up by living cells actively engaged in phagocytosis.

In this , trypan blue demonstrates the presence of phagocytosis in . These macrophages readily phagocytize and retain the foreign molecule, trypan blue.

(The other is from the kidney with uptake of the dye by the proximal convoluted tubules.)

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