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CHAPTER 7 - PERIPHERAL BLOOD
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 033 Blood Smear
TISSUE
Blood
(human)
STAIN
Wright's Stain
(mixture of methylene blue,
azure II, and eosin)
FIXATIVE
Air Dry
Methanol
IMAGE SIZE
150,720 x 81,008 pixels
45 GB
FILE SIZE
2.7 GB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 033 Blood Smear

Peripheral Blood

Blood smears are made by placing a drop of blood on one end of a slide, and using a spreader slide to disperse the blood from left to right.

  • - left side of the slide with the largest number of cells. The tight packing of cells distorts their .
  • - region where the cells are spaced far enough apart to be differentiated and counted. The is best in this zone.
  • - right side of the slide with the lowest number of cells. The of the cells is typically poor.

This scan was obtained with a 40x dry objective. Except for identifying the zone of best morphology, individual cells need to be viewed at higher magnification to examine their morphology.

See MH 033hr Blood Smear for an image of the feather edge acquired with a 60x oil immersion objective.

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