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CHAPTER 5 - CARTILAGE AND BONE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 043 Cancellous and Compact Bone
TISSUE
Parietal Skull
(human)
PREPARATION
Decalcified Bone
STAIN
Hematoxylin & Eosin
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZE
96,030 x 31,285 pixels
11 GB
FILE SIZE
230 MB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 043 Cancellous and Compact Bone

This slide contains two specimens:

  • - partial cross-section from the end of a long bone found in the extremities.
  • - cross-section of a bone from the skull cap (calvarium).

Examine the partial-cross section of the long bone.

What are the two types of of bones? [ + ]

  • - dense layer that forms the outside of a bone
  • – sponge-like meshwork of trabeculae (or spicules) that form the interior of bones
(These terms refer to their macroscopic appearance.)

Why is ther no or very few osteons in ? [ + ]

Trabeculae are usually thin enough that nutrients can diffuse through canaliculi connecting osteocytes in lacunae

Name of the organic matter of bone? [ + ]

Osteoid - unmineralized bone matrix of mostly collagen type I (15 to 20% of bone)

Major inorganic matter of bone? [ + ]

Calcium phosphates in the form of hydroxyapatite crystals (70 to 80% of bone)

Name of the that covers the external surface of bone? [ + ]

Periosteum (except for articular surfaces)

It is poorly preserved in these tissue sections.

Name of its two layers and how do they differ? [ + ]

  • Outer fibrous layer - collagen type I fibers and scattered fibroblasts
  • Inner osteogenic layer – osteoprogenitor cells that can differentiate into osteoblasts

What are Sharpey's fibers? [ + ]

Collagen fibers that extend from the periosteum into the extracellular matrix of bone that provide a strong anchoring for the periosteum, ligaments, and tendons to bone
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