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CHAPTER 15 - LIVER AND GALLBLADDER
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 127 Liver
TISSUE
Liver
(human)
STAIN
Azan
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZE
90,210 x 44,398 pixels
14.9 GB
FILE SIZE
1.2 GB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 127 Liver

Bile Canaliculi
(Azan)

The higher contrast staining of the liver with azan helps in the visualization of bile canaliculi. However, other structures tend to be more difficult to visualize.

  • - roughly hexagonal structure with a central vein at its center and six portal triads at its periphery.
    • - large venule at the center of the lobule.
    • - anastomosing plates, one cell thick, radiate outward from the central vein separated by sinusoidal capillaries and supported by reticular fibers.
    • - at the corners of each lobule (hepatic arterioles, portal venule, bile ductules and lymphatic vessels).
    • - lined with a simple cuboidal epithelium.
  • Bile Canaliculi (,) - bile is secreted into 1 to 2 µm diameter tubes formed by adjacent hepatocytes that drain into bile ductules at the portal triads.
    • Longitudinal Section - are seen as darker staining lines between adjacent hepatocytes.
    • Cross-Section - occasionally as a small dot in the center between two hepatocytes.
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