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This specimen was stained with a combination of iron hematoxylin (basic dye) & aniline blue (acidic dye; IHAB). Acidophilic structures (e.g., collagen) stain bright blue while basophilic structures stain black to brown (e.g., heterochromatin).
Mucous Neck Cells - smaller, basophilic cells found in the isthmus and neck between the lightly-stained parietal cells. Their secretion granules are stained dark-gray/black.
Parietal Cells - large, oval cells with a central nucleus found mostly in the neck, but are found throughout the gland. They are stained light-gray and do not contain secretion granules.
Chief Cells - smaller, basophilic cells found mostly in the fundus/body of the gland. Their secretion granules are stained dark-gray/black.
