Liver tissue fixed after perfusion of a dye (India ink) through the peripheral vascular system. This demonstrates the pattern of blood flow through the hepatic sinusoids.
Hepatocytes cells form radial sheets, or plates, that may be branched. These cords of hepatocytes are usually only one cell thick with two surfaces in direct contact with blood. These capillaries are called sinusoids because of discontinuities in their endothelial walls. They are easily seen to be filled with black ink.
Blood from branches of the portal vein and hepatic artery flows into the sinusoids and exits through the central veins. The ink was not retained in blood vessels in the portal triads and central veins.