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CHAPTER 13 - ENDOCRINE GLANDS
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
UCSF 295 Pancreas
TISSUE
Pancreas
(guinea pig)
STAIN
Gomori's Stain
(chromium hematoxylin-phloxine)
IMAGE SIZE
20,181 x 22,231 pixels
1.3 GB
FILE SIZE
130 MB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Medical School
University of California
San Francisco, CA

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Robert L. Sorenson, Ph.D.

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University of Minnesota
Department of Genetics, Cell Biology and Development
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321 Church St SE
Minneapolis, MN 55455

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UCSF 295 Pancreas

Islets of Langerhans

Islets of Langerhans (pancreatic islets) contain four major types of endocrine cells:

  • Beta Cells - most common and secrete insulin.
  • Alpha Cells - second most frequent and secrete glucagon.
  • Delta Cells - secrete somatostatin.
  • PP Cells - secrete pancreatic polypeptide.

Gomori's stain was an early histochemical technique that distinguished between beta- and alpha-cells. Beta cells stain blue, while alpha cells stain pink. The other types of islet cells cannot be identified.

Today, this technique is primarily of historical interest because of the availability of insulin- and glucagon-specific antibodies for immunohistochemistry.

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