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Fibrocartilage is a mixture of dense regular connective tissue and hyaline cartilage. It is found in intervertebral discs, pubic symphysis, articular discs and menisci of joints.
Intervertebral discs that are found between adjacent vertebrae of the spine contain fibrocartilage. They have a fibrous appearance of mostly collagen fibers with interspersed areas of cartilage.
(Note that the cytoplasm has been extracted from most of the cells during sample preparation.)
Collagen Fibers - the majority of the fibrocartilage is a mixture of type I and type II collagen. They type I collagen is stained pink/red.
Fibroblasts - scattered cells within fibrous regions with elongated or flattened nuclei. Few are seen in this specimen.
Chondrocytes - are dispersed between collagen fibers singularly, in columns,
or in isogenous groups and are surrounded by a basophilic matrix.
Matrix - much less material surrounds each chondrocyte than in hyaline cartilage. It is composed of type II collagen and a ground substance of proteoglycans. The basophilia is due to a high content of sulfated glycosaminoglycans (GAGs).