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CHAPTER 5 - CARTILAGE AND BONE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 040 Intervertebral Disk
TISSUE
Intervertebral Disk
(human)
STAIN
Hematoxylin & Eosin
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZE
36,860 x 42,968 pixels
5.9 GB
FILE SIZE
270 MB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 040 Intervertebral Disk

Fibrocartilage

Fibrocartilage is a mixture of dense regular connective tissue and hyaline cartilage. It is found in intervertebral discs, pubic symphysis, articular discs and menisci of joints.

Intervertebral discs that are found between adjacent vertebrae of the spine contain . They have a fibrous appearance of mostly collagen fibers with interspersed areas of cartilage.

(Note that the cytoplasm has been extracted from most of the cells during sample preparation.)

  • - the majority of the fibrocartilage is a mixture of type I and type II collagen. They type I collagen is stained pink/red.
  • - scattered cells within fibrous regions with elongated or flattened nuclei. Few are seen in this specimen.
  • Chondrocytes - are dispersed between collagen fibers , in , or in and are surrounded by a basophilic matrix.
  • - much less material surrounds each chondrocyte than in hyaline cartilage. It is composed of type II collagen and a ground substance of proteoglycans. The basophilia is due to a high content of sulfated glycosaminoglycans (GAGs).
  • There is no perichondrium.
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